Midfielder Duane Holmes has been dropped from the U.S. CONCACAF Gold Cup roster because of a reoccurrence of a left quadriceps strain, and he has been replaced by midfielder Djordje Mihailovic.

The U.S. Soccer Federation announced the change Saturday, four days after the Americans replaced injured midfielder and defender Tyler Adams with Reggie Cannion.

Holmes made his U.S. debut on June 5 in an exhibition against Jamaica.

Coming off a 1-0 loss to Jamaica and a 3-0 defeat to Venezuela, the U.S. opens its Gold Cup title defense against Guyana on Tuesday at St. Paul, Minnesota, in the Americans’ first competitive match since the October 2017 loss at Trinidad and Tobago that ended a

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