Two passengers on board ‘ Seaside ship tested positive for the coronavirus that causes COVID-19, disembarking Tuesday during a scheduled port call at Siracusa, a city in Sicily, Italy.

Both passengers were asymptomatic and tested positive during a routine test scheduled midway through the itinerary during all MSC cruises, spokesperson Paige Rosenthal told USA TODAY Wednesday.

“Immediately after testing positive, the two passengers and their traveling parties were isolated,” Rosenthal said. “Similarly, their close contacts were quickly identified and isolated.”

Passengers traveling with the two guests tested negative, as did other close contacts.

All MSC passengers are required to take a test before leaving home 48 to 96 hours before the cruise’s departure and one more before embarkation. “This was their third test,” Rosenthal said.