Stylized head silhouette. Entangled threads instead of brains. Symbol of confused consciousness.
This is the fourth article in a series focusing on cognitive dysfunction and inflammation. The first part can be read here, the second can be read here, and the third, here. For additional writings on inflammation and Covid-19, please see my website: www. williamhaseltine.com
In previous installments of this series, we looked at new research that suggests even mild SARS-CoV-2 infection can impair cognitive function. The first step in this chain of events happens when the virus sets off an intense inflammatory response that eventually reaches the brain. Once there, inflammation triggers reactivity of microglial cells —the resident immune cells of the central nervous system— which produce yet more inflammation and interfere with the normal functioning of important cells, including oligodendrocytes and neural precursor cells (Figure 1).
FIGURE 1. A schematic representation of the mechanism underlying cognitive dysfunction following … [+] mild Covid-19, as described by Fernández-Castañeda et al. (2022).
SOURCE: ACCESS Health International
But given that all of this happens off the back of inflammation, which we experience during any number of infections or injuries, wouldn’t we expect to see something similar unfold in other viral diseases? As part of their research, Fernández-Castañeda et al. asked themselves this same question. In the search for an answer, they turned to influenza.
Like Covid-19, the flu has also been associated with cognitive and neurological issues. And like SARS-CoV-2, influenza is rarely neuroinvasive — even though both viruses can infect the brain directly, as can many other viruses, it seems to happen only infrequently. It could be, then, that influenza causes cognitive issues through a similar mechanism as SARS-CoV-2.
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To find out, the researchers exposed a group of mice to the H1N1 strain of influenza. This strain is not neuroinvasive in mouse models, meaning infection is restricted to the trachea and lungs. To further keep things consistent with their Covid-19 experiments, the researchers made sure that the mice suffered only mild flu, as evidenced by minimal sickness behaviors and minimal weight loss.
Despite largely mild illness, the mice showed signs of elevated inflammation, with a noticeable increase in cytokine and chemokine levels. The researchers found these both in the blood as well as in the cerebrospinal fluid, indicating that inflammation had made its way into the central nervous system.
The influenza-induced cytokine profile closely mirrored that of mice with mild respiratory Covid-19 (Figure 2). In both cases, C-C motif chemokine ligand 11 (CCL11) was present in cerebrospinal fluid from seven days all the way to seven weeks after infection. In fact, CCL11 levels were higher at seven weeks than they were at seven days.
FIGURE 2. Cytokine/chemokine profiles following mild Covid-19 and mild flu. Notice CCL11, present in … [+] both cases seven days post infection (7DPI) as well as seven weeks post infection (7WPI).
FROM: “Mild respiratory COVID can cause multi-lineage neural cell and myelin dysregulation” Fernández-Castañeda et al. 2022
This means the first part of the mechanism driving cognitive impairment after Covid-19 —the peripheral inflammation that triggers neuroinflammation— is also present during infection with influenza. What about the remaining elements?
In the context of Covid-19, elevated CCL11 levels were associated with an increase in reactivity of microglial cells. Studying the white matter of those mice exposed to the flu, the group of researchers discovered a similar increase in microglial reactivity; although the total number of microglial cells remained the same in both the flu group and the control group, the number of activated microglial cells was noticeably higher in the mice recovering from illness.
The increase in activated microglial cells was accompanied by both damage to oligodendrocytes and by a decrease in the production of new neurons in the hippocampus, called neurogenesis. The exact same pathology seen in the Covid-19 mice.
Neurogenesis in the hippocampus was impaired as early as seven days following influenza infection, and this persisted until at least seven weeks after infection. Microglial cells in this region remained reactive throughout. Unlike the Covid-19 mice, however, those exposed to the flu showed signs of recovery to oligodendrocyte numbers. Although the …